Although the test has been designed to be a point-of-care test it can also be used as a simple, inexpensive laboratory test for smoking. We have used the test in this way with a large University department who are investigating an intervention to reduce smoking in pregnancy.

We have investigated the role of smoking in male infertility. The test results showed nicotine intake was not related to either abnormal sperm motility or morphology. This quantitive study dispelled the previous hypothesis that smoking reduced fertility by damaging sperm.

The test was also used to assess the validity of self-reported smoking in patients with diabetes. The results showed 6% of patients denied smoking.